Plant Transcription Factor Database
Previous version: v3.0
Nelumbo nucifera
HSF Family
Species TF ID Description
NNU_000255-RAHSF family protein
NNU_000625-RAHSF family protein
NNU_002039-RAHSF family protein
NNU_002776-RAHSF family protein
NNU_003323-RAHSF family protein
NNU_003516-RAHSF family protein
NNU_005975-RAHSF family protein
NNU_006787-RAHSF family protein
NNU_007159-RAHSF family protein
NNU_008978-RAHSF family protein
NNU_010743-RAHSF family protein
NNU_011634-RAHSF family protein
NNU_011653-RAHSF family protein
NNU_015568-RAHSF family protein
NNU_016429-RAHSF family protein
NNU_020109-RAHSF family protein
NNU_020992-RAHSF family protein
NNU_021222-RAHSF family protein
NNU_021638-RAHSF family protein
NNU_021728-RAHSF family protein
NNU_022682-RAHSF family protein
NNU_022718-RAHSF family protein
NNU_022792-RAHSF family protein
NNU_023010-RAHSF family protein
NNU_023191-RAHSF family protein
NNU_024675-RAHSF family protein
NNU_025342-RAHSF family protein
NNU_025540-RAHSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645